Previous studies indicate that a direct detection of testosterone esters in both hair and plasma is possible.Aim of the proposed project is the investigation and optimisation of the direct detection of testosterone esters in body fluids like serum, whole blood and stabilized blood with an already developed detection method using modern and sensitive technology. Beside the difficulty of their detection in urine samples, these substances are used for positive effects on mood states, and also to lower the level of fatigue. Although knowledge of androgen steroid metabolism has increased during the past decades and analytical guidance has been provided by sport authorities, detection of doping with testosterone remains a challenge in sport. This compound is prohibited by sport authorities because its administration will lower the urinary T/E ratio, a marker of testosterone administration.19 This substance was first listed in the IOC doping list in 2001 as a steroid precursor. This steroid metabolite was first listed in the WADA Prohibited List in 2004 as a marker of endogenous anabolic androgenic steroid administration. This substance was first listed in the WADA Prohibited List in 2004 as an anabolic steroid. Information on some of the prohibited substances on the World Anti-Doping Agency Prohibited list. Therapeutic use exemption (TUE) is a term used by WADA and the United States Anti-Doping Agency to denote banned substances that athletes may be "required to take to treat an illness or condition". Fluoxymesterone (trade name Halotestin) is an anabolic steroid with strong androgenic properties that has been used in the treatment of male hypogonadism, delayed puberty in males, and in the treatment of breast neoplasms in women. This substance was first referenced in WADA documents from 2004 as a urinary marker in steroid detection protocols. DMT (desoxy-methyl-testosterone) this was the second designer steroid uncovered. Turinabol was the key steroid administered to approximately 10,000 athletes from East Germany (GDR). Clostebol is the 4-chloro derivative of the natural hormone testosterone.It is a weak anabolic Steroid used often by Germans during their Olympic glory days. Clostebol, usually as the ester clostebol acetate, is a synthetic anabolic androgenic steroid. Because of this the drug was re-classified for 2011 from the 'non-specified' to 'specified' stimulant class, meaning that if the athlete can prove that they did not take the drug intentionally he or she may get a reduced sanction. The World Anti-Doping Agency has recently clarified that glycerol is prohibited as a plasma expander which requires the ingestion of quantities far beyond that which are commonly found in foodstuffs and toiletries. This brand name for fluticasone was first documented in IOC/WADA contexts around 2001 as an inhaled corticosteroid allowed under medical declaration. Aging triathletes face unique challenges in maintaining performance and health. It is important to recognize the impact of energy availability on the overall well-being and athletic performance of aging athletes, as hormonal imbalances may not be the primary cause of their symptoms. Keay further explains that RED-S encompasses various physiological and psychological effects that result from prolonged LEA. Hormone health expert Dr. Nicky Keay says many symptoms in aging athletes are more likely due to Low Energy Availability (LEA) or Relative Energy Deficiency in Sport (RED-S), not age-related hormonal decline.